R6G phosphoramidite, 6-isomer, CAS [[1355330-47-0]]

Product Overview

• Article Name: R6G phosphoramidite, 6-isomer, CAS [[1355330-47-0]]
• Biozol Catalog Number: ABC-A270259-5
• Supplier Catalog Number: A270259-5
• Alternative Catalog Number: ABC-A270259-5
• Manufacturer: Antibodies.com
• Category: Biochemikalien

Product Description

R6G phosphoramidite for oligonucleotide synthesis, pure 6-isomer. R6G (rhodamine 6G) is a xanthene dye of the rhodamine family with high fluorescence quantum yield and high molar extinction coefficient. Relative to those of fluorescein, the absorption (518 nm) and emission (542 nm) maxima of 6-R6G are shifted into the long-wave region. R6G can be used as a FRET acceptor in DNA-sequencing and in short tandem repeat (STR) analysis. The dye is commonly used for producing TaqMan probes for quantitative PCR. In comparison with HEX and JOE dyes, R6G in combination with BHQ1 quencher has stronger quenching in molecular beacon probes, significantly reducing background fluorescence in PCR. Coupling time of 10 minutes. Deprotection should be performed in a solution of tert-Butylamine in water (1:3 v/v) overnight at 55C. Aqueous ammonia and AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v) must NOT be used for deprotection of the modified oligonucleotide from the solid-phase support because of complete and irreversible R6G degradation.

Product Properties

• Molecular Weight: 949.93 Da
• Purity: > 95% (by 1H and 31P NMR, and HPLC-MS). Isomeric purity > 97%.
• Form: Yellowish foam.
• CAS Number: [1355330-47-0]
• Formula: C46H54F6N5O8P