Monoclonal Antibody to CD33(Clone: ABM29D3)

Product Overview

• Article Name: Monoclonal Antibody to CD33(Clone: ABM29D3)
• Biozol Catalog Number: ABI-10-4064-25
• Supplier Catalog Number: 10-4064-25
• Alternative Catalog Number: ABI-10-4064-25-25UG
• Manufacturer: Abeomics
• Host: Mouse
• Category: Antikörper
• Application: FACS, IHC, WB
• Species Reactivity: Human
• Immunogen: A partial length recombinant CD33 protein (amino acids 28-320) was used as the immunogen for this antibody.
• Alternative Names: CD33||SIGLEC3

Product Description

CD33 is a member of the SIGLEC (Sialic Acid-Binding Ig-Like Lectin) family of receptors, and the gene comprises seven coding exons. Exon 2 encodes the canonical IgV domain, exon 4 encodes the IgC structural domain, and exons 6 and 7 encode cytosolic ITIMs (Immunotyrosine Inhibitory Motifs). CD33 acts as a cell surface antigen which is expressed on normal myeloid cells and CD34+ blasts in AML (Acute Myeloid Leukemia). The antigen serves as a target of GO (Gemtuzumab/Ozogamicin), which exerts anti-leukemic effects in refractory AML.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [ABM29D3]
• NCBI: 945
• UniProt: P20138
• Purity: Protein G Chromatography
• Form: Purified
• Target: CD33

Application Notes

• Application Dilute: Western blot analysis: 2-4 µg/ml, Immunohistochemical analysis: 5 µg/ml FACS: 0.2-0.5 µg/10 6 cells

Images

Fig-5: Cell Surface flow analysis of hCD33 in PBMC (Monocytes) using 0.2µg/10 6 cells of CD33 clone ( ABM29D3). Green represents isotype control, red represents anti-hCD33 antibody. Goat anti-mouse PE conjugated secondary antibody (ABEOMICS) was used.

Fig-1: Immunohistochemical analysis of CD33 in adenocarcinoma of rectum using CD33 antibody (Clone: ABM29D3) at 5 µg/ml.

Fig-3: Immunohistochemical analysis of CD33in Renal Cell Carcinoma using CD33 antibody (Clone: ABM29D3) at 5 µg/ml.

Fig-4: Immunohistochemical analysis of CD33in squamous cell carcinoma of esophagus using CD33 antibody (Clone: ABM29D3) at 5 µg/ml.

Fig-2: Western blot analysis of CD33. Anti- CD33 antibody (Clone: ABM29D3) was used at 2 µg/ml on Human Spleen and DU145 lysates.