Monoclonal Antibody to RIG-I (Clone: ABM4H29)

Product Overview

• Article Name: Monoclonal Antibody to RIG-I (Clone: ABM4H29)
• Biozol Catalog Number: ABI-10-4121
• Supplier Catalog Number: 10-4121
• Alternative Catalog Number: ABI-10-4121-100UG
• Manufacturer: Abeomics
• Host: Mouse
• Category: Antikörper
• Application: FACS, IHC, WB
• Species Reactivity: Human
• Immunogen: A partial length recombinant human RIG-I protein (amino acids 1-220) was used as the immunogen for this antibody.
• Alternative Names: DDX58

Product Description

RIG-I (retinoic-acid-inducible gene I), also known as DDX58 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 58) is a 925-residue cytoplasmic viral RNA receptor, critically involved in the activation of the innate immune response to RNA virus infection. It is a member of the RIG-I-like receptor (RLR) family and is an essential intracellular sensor for several 5-triphosphorylated RNA viruses. RIG-I elicits its antiviral interferon (IFN) responses by recognizing viral double-stranded RNAs (dsRNAs). Structurally it comprises a helicase domain, a C-terminal domain, and N-terminal CARDs (caspase activation recruitment domains) involved in activating MAVS (mitochondrial antiviral signaling protein). Upon binding of 5-triphosphorylated RNA, RIG-I undergoes conformational changes and post-translational modifications that allow multimerization and interaction with the mitochondrial antiviral signaling protein (MAVS).

Product Properties

• Clonality: Monoclonal
• Clone Designation: [ABM4H29]
• NCBI: 23586
• UniProt: O95786
• Purity: Protein G Chromatography
• Form: Purified
• Target: RIG-I

Application Notes

• Application Dilute: Western blot analysis: 2-4 µg/ml, Immunohistochemical analysis: 15 µg/ml, Flowcytometric analysis: 0.5-1µg/10 6 cells

Images

Fig-1: Western blot analysis of RIG1/DDx58. Anti-RIG1/DDx58 antibody (Clone: ABM4H29) was tested at 2 µg/ml on human kidney lysate.

Fig-2: Immunohistochemical analysis of RIG1/DDx58 in human Colon Tissue using RIG1/DDx58 antibody (Clone: ABM4H29) at 15 µg/ml.

Fig-3: Immunohistochemical analysis of RIG1/DDx58 in human Spleen Tissue using RIG1/DDx58 antibody (Clone: ABM4H29) at 15 µg/ml.

Figure-4: Intracellular flow cytometric analysis of RIG-I in K562 Cell line using 0.5 µg/10 6 cells of Anti-RIGI antibody (ABM4H29). Green represent isotype control and red represent Anti-RIG I antibody (10-4121 Abeomics). Goat anti-mouse PE conjugate was used as secondary.