Anti-Influenza A, HA (Clone: Flu-5J8) Purified No Carrier Protein, Clone: [Flu-5J8], Monoclonal

Product Overview

• Article Name: Anti-Influenza A, HA (Clone: Flu-5J8) Purified No Carrier Protein, Clone: [Flu-5J8], Monoclonal
• Biozol Catalog Number: ABI-12-8439
• Supplier Catalog Number: 12-8439
• Alternative Catalog Number: ABI-12-8439-1MG
• Manufacturer: Abeomics
• Category: Antikörper
• Application: ELISA
• Alternative Names: Flu

Product Description

Specificity: Flu-5J8 activity is directed against a conserved H1 epitope adjacent to the receptor binding site domain on the HA globular head. Furthermore, clone Flu-5J8 binds HA using receptor mimicry3. By inserting its HCDR3 into the HA receptor binding site (RBS), Flu-5J8 closely mimics the natural sialoglycan receptor and blocks viral-host interactions. Flu-5J8 contacts the Sb and Ca2 antigenic sites and binds to conserved residues in the RBS (130 loop, 190 helix, and 220 loop) as well as outside the RBS at the 140 loop. Furthermore, EM reconstructions show that Flu-5J8 occupies all three potential binding sites at the HA apex at and around the RBS. Mutations located between the receptor-binding pocket and the Ca2 antigenic site at residues 133A, 137, or 222 eliminate binding. In a lethal challenge in mice, Flu-5J8 protected all animals at the high and medium doses given and reduced lung virus titers relative to the control2. Antigen Distribution: HA is on the viral surface. Background: Hemagglutinin (HA) is a glycoprotein on the Influenza A (IAV) viral surface1. HA consists of two domains: an antigenically variable head and a more conserved stem. There are 18 HA subtypes. Neutralizing antibodies targeting the head domain are typically restricted to within subtype, while antibodies targeting the stem offer broader protection. In contrast, Flu-5J8 targets the HA globular head and offers broad protection2. Flu-5J8 inhibits all 1918 to 1977 H1N1 strains tested plus the pandemic 2009 strain but not the seasonal H1N1 strains from 1999 or 2007.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [Flu-5J8]
• Purity: Purity :>=90% monomer by analytical SEC and SDS-PagePreparation : Recombinant antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
• Target: Flu A, HA

Application Notes

• Application Dilute: ELISA FC N