iNOS Leeporter(TM) Luciferase Reporter-RAW264.7 Cell Line Preis auf Anfrage

Product Overview

• Article Name: iNOS Leeporter(TM) Luciferase Reporter-RAW264.7 Cell Line Preis auf Anfrage
• Biozol Catalog Number: ABI-14-115ACL
• Supplier Catalog Number: 14-115ACL
• Alternative Catalog Number: ABI-14-115ACL-1VIAL
• Manufacturer: Abeomics
• Category: Zellen/Zellkultur
• Application: FA

Product Description

The iNOS Leeporter(TM) Luciferase Reporter cell line is a stably transfected RAW264.7 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the iNOS promoter. Inducible nitric oxide synthase (iNOS) is an inducible enzyme that catalyzes the production of nitric oxide (NO) from L-arginine. NO is one of the smallest signaling molecules that can diffuse into the cell and is involved in various physiological functions, pathogenesis of septic shock, many diseases associated with autoimmunity, and tumorigenesis. iNOS gene is generally known to be induced by various proinflammatory cytokines and pathogen-associated molecular patterns such as Toll-like receptor (TLR) ligands. The iNOS induction by lipopolysaccharide (LPS), the TLR4 ligand, is shown in Figure 1.

Application Notes

• Application Dilute: Application: Monitor the iNOS induction activity. Screen for activators or inhibitors of the iNOS signaling pathway. Culture conditions: Cells should be grown at 37C with 5% CO2 using DMEM medium (w/ L-Glutamine, 4.5g/L Glucose and Sodium Pyruvate) supple