HRE Leeporter(TM) Luciferase Reporter-HeLa Cell Line Preis auf Anfrage
Biozol Catalog Number:
ABI-14-118ACL
Supplier Catalog Number:
14-118ACL
Alternative Catalog Number:
ABI-14-118ACL-1VIAL
Manufacturer:
Abeomics
Category:
Zellen/Zellkultur
Application:
FA
The HRE Leeporter(TM) Luciferase Reporter cell line is a stably transfected HeLa cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the hypoxia response element (HRE). In response to hypoxia (low oxygen), HREs of target genes are recognized and regulated by the hypoxia-inducible factors (HIFs) which belong to the family of basic helix-loop-helix transcription factors and form heterodimeric complex comprising the alpha subunit (HIF-1 alpha, HIF-2 alpha and HIF-3 alpha) and beta subunit (Arnt1, Arnt2 and Arnt3), among which HIF-1 alpha and HIF-2 alpha are predominant isoforms. Activation of HIFs can also be mediated by chemical hydroxylase inhibitors as hypoxia mimetics including the iron chelator desferrioxamine and cobalt chloride.The HRE induction by cobalt chloride is shown in Figure 1.
Application Dilute:
Application: Monitor the HIF induction activity. Screen for activators or inhibitors of the hypoxia signaling pathway. Culture conditions: Cells should be grown at 37C with 5% CO2 using DMEM medium (w/ L-Glutamine, 4.5g/L Glucose and Sodium Pyruvate) supp
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