VISTA Stable Cell Line-H is a stably transfected HEK293 cell line which expresses human V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA, also known as VSIR, B7-H5, PD-1H, and SISP1). Sequence data: hVISTA (accession number NP_071436) MGVPTALEAGSWRWGSLLFALFLAASLGPVAAFKVATPYSLYVC PEGQNVTLTCRLLGPVDKGHDVTFYKTWYRSSRGEVQTCSERRPIRNLTFQDLHLHHG GHQAANTSHDLAQRHGLESASDHHGNFSITMRNLTLLDSGLYCCLVVEIRHHHSEHRV HGAMELQVQTGKDAPSNCVVYPSSSQDSENITAAALATGACIVGILCLPLILLLVYKQ RQAASNRRAQELVRMDSNIQGIENPGFEASPPAQGIPEAKVRHPLSYVAQRQPSESGR HLLSEPSTPLSPPGPGDVFFPSLDPVPDSPNFEVI
Application Dilute:
Application:. Screen for antibodies of human VISTA through Flow Cytometry. Culture conditions: Cells should be grown at 37C with 5% CO2 using DMEM medium (w/ L-Glutamine, 4.5g/L Glucose and Sodium Pyruvate) supplemented with 10% heat-inactivated FBS and 1
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