TLR1/HEK293 Stable Cell Line is a stably transfected HEK293 cell line which expresses human Toll-like receptor 1 (TLR1, also designated as CD281). TLR1 forms a heterodimer with TLR2, and then the TLR1-TLR2 heterodimer responds to triacylated lipoproteins from Gram-negative bacteria and mycoplasma, which leads to the NF-kB-induced pro-inflammatory cytokine production and activation of innate immune responses. Note that TLR1 in the TLR1/HEK293 stable cell line contains the N-terminal HA tag (Figure 1). Sequence data: Human TLR1 (accession number NP_003254) MTSIFHFAIIFMLILQIRIQLSEESEFLVDRSKNGLIHVPKDLS QKTTILNISQNYISELWTSDILSLSKLRILIISHNRIQYLDISVFKFNQELEYLDLSH NKLVKISCHPTVNLKHLDLSFNAFDALPICKEFGNMSQLKFLGLSTTHLEKSSVLPIA HLNISKVLLVLGETYGEKEDPEGLQDFNTESLHIVFPTNKEFHFILDVSVKTVANLEL SNIKCVLEDNKCSYFLSILAKLQTNPKLSNLTLNNIETTWNSFIRILQLVWHTTVWYF SISNVKLQGQLDFRDFDYSGTSLKALSIHQVVSDVFGFPQSYIYEIFSNMNIKNFTVS GTRMVHMLCPSKISPFLHLDFSNNLLTDTVFENCGHLTELETLILQMNQLKELSKIAE MTTQMKSLQQLDISQNSVSYDEKKGDCSWTKSLLSLNMSSNILTDTIFRCLPPRIKVL DLHSNKIKSIPKQVVKLEALQELNVAFNSLTDLPGCGSFSSLSVLIIDHNSVSHPSAD FFQSCQKMRSIKAGDNPFQCTCELGEFVKNIDQVSSEVLEGWPDSYKCDYPESYRGTL LKDFHMSELSCNITLLIVTIVATMLVLAVTVTSLCSYLDLPWYLRMVCQWTQTRRRAR NIPLEELQRNLQFHAFISYSGHDSFWVKNELLPNLEKEGMQICLHERNFVPGKSIVEN IITCIEKSYKSIFVLSPNFVQSEWCHYELYFAHHNLFHEGSNSLILILLEPIPQYSIP SSYHKLKSLMARRTYLEWPKEKSKRGLFWANLRAAINIKLTEQAKK
Application Dilute:
Application:. Functional assay. Culture conditions: Cells should be grown at 37oC with 5% CO2 using DMEM medium (w/ L-Glutamine, 4.5g/L Glucose and Sodium Pyruvate) supplemented with 10% heat-inactivated FBS and 1% Pen/Strep, plus 10 µg/ml of Blasticidin.
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