STAT3 Leeportera GFP Reporter-HEK293 Cell Line Preis auf Anfrage
Biozol Catalog Number:
ABI-14-702ACL
Supplier Catalog Number:
14-702ACL
Alternative Catalog Number:
ABI-14-702ACL-1VIAL
Manufacturer:
Abeomics
Category:
Zellen/Zellkultur
Application:
FA
The STAT Leeporter(TM) GFP Reporter cell line is a stably transfected HEK293 cell line, which expresses enhanced green fluorescent protein (eGFP) reporter gene under the transcriptional control of the STAT3 responsive promoter, so that the cell line is designed to measure the transcriptional activity of STAT3. As a transcription factor, Signal Transducer and Activator of Transcription 3 (STAT3) is activated through phosphorylation at tyrosine 705 in response to various cytokines including IL-6, interferons, epidermal growth factor, hepatocyte growth factor and leukemia inhibitory factor. The phosphorylated STAT3 forms homodimers or heterodimers with STAT1, and the dimers translocate to nucleus in which DNA binding/promoter induction occurs. The cell line is designed to analyze by fluorescence microscopy and flow cytometry. The STAT3 induction by IL-6 is shown in Figures 1 and 2.
Application Dilute:
Application: Monitor the STAT3 signaling pathway activity. Screen for activators or inhibitors of the STAT3 signaling pathway. Culture conditions: Cells should be grown at 37oC with 5% CO2 using DMEM medium (w/ L-Glutamine, 4.5g/L Glucose and Sodium Pyruv
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