TET-ON Leeportera GFP Reporter-HEK293 Cell Line Preis auf Anfrage
Biozol Catalog Number:
ABI-14-703ACL
Supplier Catalog Number:
14-703ACL
Alternative Catalog Number:
ABI-14-703ACL-1VIAL
Manufacturer:
Abeomics
Category:
Zellen/Zellkultur
Application:
FA
The TET-ON Leeporter(TM) GFP Reporter cell line is a stably transfected HEK293 cell line, which expresses enhanced green fluorescent protein (eGFP) reporter gene under the transcriptional control of the Tetracycline-inducible promoter. So the GFP expression can be turned on by treating the cell line with doxycycline which can regulate the level of GFP expression in the cells, while the resting cells do not express GFP. The cell line is designed to analyze by fluorescence microscopy and flow cytometry. The GFP expression in the cell line by doxycycline is shown in Figures 1 and 2.
Application Dilute:
Application: Monitor the tetracycline (doxycycline)-controlled GFP expression. Tetracycline activity. Culture conditions: Cells should be grown at 37oC with 5% CO2 using DMEM medium (w/ L-Glutamine, 4.5g/L Glucose and Sodium Pyruvate) supplemented with 10
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