TET-OFF Leeportera GFP Reporter-HEK293 Cell Line Preis auf Anfrage
Biozol Catalog Number:
ABI-14-704ACL
Supplier Catalog Number:
14-704ACL
Alternative Catalog Number:
ABI-14-704ACL-1VIAL
Manufacturer:
Abeomics
Category:
Zellen/Zellkultur
Application:
FA
The TET-OF Leeporter(TM) GFP Reporter cell line is a stably transfected HEK293 cell line, which expresses enhanced green fluorescent protein (eGFP) reporter gene under the control of the Tet-Off regulatory mechanism. So the cell line can express GFP only when doxycycline is removed from the cell culture medium, and the GFP expression can be turned off by adding doxycycline to the cell culture medium. The cell line is designed to analyze by fluorescence microscopy and flow cytometry. The GFP expression patterns in the presence or absence of doxycycline are shown in Figures 1 and 2.
Application Dilute:
Application: Monitor the tetracycline (doxycycline)-controlled GFP expression. Tetracycline activity. Culture conditions: Cells should be grown at 37oC with 5% CO2 using DMEM medium (w/ L-Glutamine, 4.5g/L Glucose and Sodium Pyruvate) supplemented with 10
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