Source: Escherichia Coli.Sterile Filtered clear solution.HPRT1 has a main part in the generation of purine nucleotides through the purine salvage pathway. HPRT1 primarily functions to salvage purines from degraded DNA to renewed purine synthesis. Therefore, it performs as a catalyst in the reaction between guanine and phosphoribosyl pyrophosphate to form GMP.HPRT1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 238 amino acids (1-218 a.a) and having a molecular mass of 26.7kDa. HPRT1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Purity:
Greater than 95.0% as determined by SDS-PAGE.
Application Dilute:
Specific activity is > 15 units/mg and is defined as the amount of enzyme that catalyze the formation of 1 umole of guanosine 5a€(TM)-monophosphate (GMP) per minute from guanine and phosphoribosyl pyrophosphate at pH 7.5 at 37C.
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