Polyclonal Antibody to NF-KB-p100/p52(Phospho-Ser866), Rabbit
Biozol Catalog Number:
ABI-35-1014
Supplier Catalog Number:
35-1014
Alternative Catalog Number:
ABI-35-1014-100UL
Manufacturer:
Abeomics
Host:
Rabbit
Category:
Antikörper
Application:
IF, IHC, WB
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Peptide sequence around phosphorylation site of serine 866(E-D-S(p)-A-Y) derived from Human NF-KB-p100.
Alternative Names:
p52|| p105|| H2TF1|| LYT10|| NF-kB2
NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NF-KB1/p105, NF-KB1/p50, REL and NF-KB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. In a non-canonical activation pathway, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NF-KB2/p100 associated with RelB, inducing its proteolytic processing to NF-KB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. The NF-kappa-B heterodimeric RelB-p52 complex is a transcriptional activator. The NF-kappa-B p52-p52 homodimer is a transcriptional repressor. NF-KB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5-GGRNNYYCC-3, located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form, the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. Dobrzanski P., Ryseck R.P., Bravo R.EMBO J. 13:4608-4616(1994) Beg A.A., Baldwin A.S. Jr.Oncogene 9:1487-1492(1994)
