Anti-Human Nucleolar Antigen (Marker For Human Cells) Monoclonal Antibody(Clone: NM95)-CF488, Clone: [NM95], Mouse
Biozol Catalog Number:
ABI-36-3757
Supplier Catalog Number:
36-3757
Alternative Catalog Number:
ABI-36-3757-0.5AT100MLATUG/ML
Manufacturer:
Abeomics
Host:
Mouse
Category:
Antikörper
Application:
FACS, IF
Species Reactivity:
Human
Immunogen:
Nuclei of myeloid leukemia biopsy cells
Conjugation:
CF488
This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb NM95 recognizes an antigen associated with the nucleoli in human cells. It can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
Clonality:
Monoclonal
Clone Designation:
[NM95]
Antibody Type:
Monoclonal Antibody
Application Dilute:
Flow Cytometry (5ul per test per one million cells in 0.1ml or 5ul per 100ul of whole blood),Immunofluorescence (1:50-1:100),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC
Fig. 1: Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CF488-labeled human Nucleolar Monoclonal Antibody (NM95).
Fig. 2: Flow Cytometric Analysis of human Nucleolar Ag on 293T cells. Black: cells alone, Grey: Isotype Control, Green: CF488-labeled human Nucleolar Monoclonal Antibody (NM95).
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