It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Clonality:
Monoclonal
Clone Designation:
[BU20a]
Antibody Type:
Monoclonal Antibody
Application Dilute:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) [For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with
Fig. 1: Formalin-fixed, paraffin-embedded Mouse Small Intestine stained with BrdU Monoclonal Antibody (BU20a).
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