Anti-Cytokeratin, pan (Epithelial Marker) Monoclonal Antibody(Clone: PCK/3150), Clone: [PCK/3150], Mouse

Product Overview

• Article Name: Anti-Cytokeratin, pan (Epithelial Marker) Monoclonal Antibody(Clone: PCK/3150), Clone: [PCK/3150], Mouse
• Biozol Catalog Number: ABI-36-3777
• Supplier Catalog Number: 36-3777
• Alternative Catalog Number: ABI-36-3777-100UG
• Manufacturer: Abeomics
• Host: Mouse
• Category: Antikörper
• Application: FACS, IF, IHC
• Species Reactivity: Human
• Immunogen: Crude cytokeratin extract prepared from RT-4 and MCF-7 cells.
• Alternative Names: K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39

Product Description

Anti-cytokeratin clone PCK/3150 demonstrates a broad spectrum of cytokeratin reactivity. In normal tissues, PCK/3150 is reactive with most epithelial types, including bile ducts and hepatocytes in liver, bladder epithelium, breast ducts, bronchial epithelium, endometrium, intestinal epithelium of stomach, duodenum, ileum, colon, rectum, pancreas, ovarian epithelium, pancreatic acini, pituitary acini, pneumocytes, prostate, thyroid, skin (positive on the basal layer and negative on the superficial layers of squamous epithelium), and apocrine and sweat glands. In tumors, PCK/3150 is reactive with most carcinomas, including breast, transitional cell (TCC), renal cell (RCC), lung adenocarcinoma, lung small cell, lung squamous cell, endometrial, prostate, ovarian, hepatocellular (HCC), colorectal CA, stomach and thyroid. It is negative in certain normal tissues, including brain, lymphocytes and all cells of hematolymphoid origin, muscle, brain, nerves, endothelium and in certain tumors including most melanomas, sarcomas, lymphomas, primitive neuroectodermal tumors (PNET)/Ewings and gastrointestinal stromal tumors (GIST). Positivity has been seen on some dendritic cells in lymph nodes, some endothelia, and some muscle cells.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [PCK/3150]
• Antibody Type: Monoclonal Antibody

Application Notes

• Application Dilute: Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml),,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 9

Images

Fig. 1: Formalin-fixed, paraffin-embedded human Prostate stained with Pan-Cytokeratin Mouse Monoclonal Antibody (PCK/3150).

Fig. 2: Formalin-fixed, paraffin-embedded human Prostate stained with Pan-Cytokeratin Mouse Monoclonal Antibody (PCK/3150).

Fig. 3: Immunofluorescence staining of HeLa cells using Pan-Cytokeratin Mouse Monoclonal Antibody (PCK/3150) followed by goat anti-Mouse IgG conjµgated to CF488 (green). Nucleus stained with Reddot (Red).

Fig. 4: SDS-PAGE Analysis Purified Pan-Cytokeratin Mouse Monoclonal Antibody (PCK/3150). Confirmation of Purity and Integrity of Antibody.

Fig. 5: Flow Cytometric Analysis of HeLa cells using Pan-Cytokeratin Mouse Monoclonal Antibody (PCK/3150) followed by goat anti-Mouse IgG-CF488 (Blue), Isotype Control (Red).