Human MIF (Macrophage migration inhibitory factor) Pre-Coated ELISA kit
Biozol Catalog Number:
ABI-90-2010
Supplier Catalog Number:
90-2010
Alternative Catalog Number:
ABI-90-2010-1X96TESTS
Manufacturer:
Abeomics
Category:
Sonstiges
Application:
ELISA
This assay employs an antibody specific for Anti-human MIF coated on a 96-well plate. Standards and samples are pipetted into the wells and MIF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MIF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Application Dilute:
This immunoassay kit allows for the in vitro quantitative determination of Human MIF concentrations in serum, plasma and other biological fluids. Detection Range: 0.781 - 50 ng/ml Sensitivity: < 0.469 ng/ml
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