Human 15-LO (Arachidonate 15-Lipoxygenase) Pre-Coated ELISA Kit

Product Overview

• Article Name: Human 15-LO (Arachidonate 15-Lipoxygenase) Pre-Coated ELISA Kit
• Biozol Catalog Number: ABI-90-2096
• Supplier Catalog Number: 90-2096
• Alternative Catalog Number: ABI-90-2096-1X96TESTS
• Manufacturer: Abeomics
• Category: Sonstiges
• Application: ELISA

Product Description

This assay employs an antibody specific for Anti-human 15-LO coated on a 96-well plate. Standards and samples are pipetted into the wells and 15-LO present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human 15-LO antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of 15-LO bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Application Notes

• Application Dilute: This immunoassay kit allows for the in vitro quantitative determination of Human 15-LO concentrations in serum, plasma and other biological fluids. Detection Range: 62.5 - 4000 pg/ml Sensitivity: < 37.5 pg/ml