Human PD-1 (Programmed Cell Death Protein1) Pre-Coated ELISA Kit

Product Overview

• Article Name: Human PD-1 (Programmed Cell Death Protein1) Pre-Coated ELISA Kit
• Biozol Catalog Number: ABI-90-2111
• Supplier Catalog Number: 90-2111
• Alternative Catalog Number: ABI-90-2111-1X96TESTS
• Manufacturer: Abeomics
• Category: Sonstiges
• Application: ELISA

Product Description

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human PD-1 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human PD-1 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human PD-1 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450 nm in a microplate reader. Concentration of Human PD-1 can be calculated using the standard curve.

Application Notes

• Application Dilute: This immunoassay kit allows for the in vitro quantitative determination of Human PD-1 concentrations in serum, plasma and other biological fluids. Detection Range: 0.156 - 10 ng/ml Sensitivity: < 0.094 ng/ml