Human TIMP-1 (Metalloproteinase Inhibitor-1) Pre-Coated ELISA Kit

Catalog Number: ABI-90-2124
Article Name: Human TIMP-1 (Metalloproteinase Inhibitor-1) Pre-Coated ELISA Kit
Biozol Catalog Number: ABI-90-2124
Supplier Catalog Number: 90-2124
Alternative Catalog Number: ABI-90-2124-1X96TESTS
Manufacturer: Abeomics
Category: Sonstiges
Application: ELISA
This assay employs an antibody specific for Anti-human TIMP-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIMP-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human TIMP-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Application Dilute: This immunoassay kit allows for the in vitro quantitative determination of Human TIMP-1 concentrations in serum, plasma and other biological fluids. Detection Range: 31.2 - 2000 pg/ml Sensitivity: < 18.75 pg/ml