This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-canine IL-12/p70 antibody was pre-coated into 96-well plates. Biotin conjugated anti-canine IL-12/p70 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the canine IL-12/p70 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450nm in a microplate reader. Concentration of Canine IL-12/p70 can be calculated using the standard curve.
Application Dilute:
This immunoassay kit allows for the in vitro quantitative determination of Canine IL-12/p70 concentrations in serum, plasma and other biological fluids. Detection Range: 15.625 - 1000 pg/ml Sensitivity: < 9.375 pg/ml
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