HotTaq PCR mix is a prepared solution containing everything needed for a successful PCR reaction except specific primers and DNA templates. The mix includes high quality recombinant Taq DNA polymerase, and nucleotides in a specifically optimized buffer formulation. For reaction set-up add the PCR Mix (25 ul) to the primers, template and water (is provided in the set, total volume of 50 ul). PCR Mix is provided with MgCl2 in the solution.
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