HepG2 (human hepatoblastoma) whole cell lysate (non-denatured), Human
Biozol Catalog Number:
ABN-L074V2
Supplier Catalog Number:
L074V2
Alternative Catalog Number:
ABN-L074V2-200
Manufacturer:
Abnova
Host:
Human
Category:
Zellen/Zellkultur
Application:
IP, WB
Species Reactivity:
Human
Whole cell lysate (non-denatured).
Buffer:
In modified RIPA Lysis Buffer.
Source:
Liver
Application Dilute:
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Application Notes:
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Mi
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