HepG2 (human hepatoblastoma) nuclear extract lysate (denatured), Human
Biozol Catalog Number:
ABN-L074V3
Supplier Catalog Number:
L074V3
Alternative Catalog Number:
ABN-L074V3-50
Manufacturer:
Abnova
Host:
Human
Category:
Zellen/Zellkultur
Application:
WB
Species Reactivity:
Human
Nuclear extract cell lysate (denatured).
Buffer:
In ready-to-use 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue).
Source:
Liver
Application Dilute:
The lysate is ready to load on SDS-PAGE for Western blotting.
Application Notes:
Nuclear extract was prepared by using a modified protocol of Dignam et al. Cells were Harvested and homogenized in Buffer A, and then centrifugated at 25,000 g for 20 minutes to remove cytoplasm and pellet the nuclei. The pellet was re-suspended in Buffe
* VAT and and shipping costs not included. Errors and price changes excepted
