mRNA Cap 2-O-methyltransferase was derived from a recombinant E.coli strain that carries the gene for the vaccinia mRNA Cap 2-O-Methyltransferase. This enzyme adds a methyl group at the 2-O position of the first nucleotide adjacent to the cap structure at the 5 end of the RNA. The enzyme utilizes S-adenosylmethionine (SAM) as a methyl donor to methylate capped RNA (cap-0) resulting in a cap-1 structure. The Cap 1 structure can increase the translation efficiency, improving the expression of mRNA in transfection and microinjection experiments. This enzyme specifically requires RNA with an m7GpppN cap as substrate. It cannot utilize RNA with pN, ppN, pppN or GpppN at the 5end. Capped RNA may be prepared via in vitro transcription using cap analog or through enzymatic capping using the Vaccinia Capping Enzyme.
Concentration:
50 U/µL
Buffer:
20 mM Tris-HC (lpH 8.0), 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.1% Triton X-100, 50% glycerol.
Form:
Liquid
Application Dilute:
In Vitro TranscriptionThe optimal working dilution should be determined by the end user.
* VAT and and shipping costs not included. Errors and price changes excepted
