T4 RNA Ligase is an ATP-dependent T4 RNA ligase (T4 RNA Ligase I) recombinantly expressed by E.coli. This enzyme catalyzes the formation of phosphodiester bonds between oligonucleotides, single-stranded RNA and DNA intermolecular/intra-molecular 5-PO4 and 3-OH.
Concentration:
10 U/µL
Buffer:
50 mM KCl, 10 mM Tris-HCl, 1 mM DTT, 0.1mM EDTA, 50 % Glycerol, (pH 7.4)
Purity:
95%
Form:
Liquid
Application Dilute:
In Vitro TranscriptionThe optimal working dilution should be determined by the end user.
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