The Small RNA Prestain Marker Plus consists of six prestained single-strand (blue and red) nucleic acids (apparent molecular weights are 20, 30, 40, 50, 75 and 100 bases) and it is visible during electrophoresis. The Small RNA Prestain Marker Plus is suitable for monitoring denaturing polyacrylamide gel electrophoresis and blotting onto membranes. The apparent sizes of bands in Small RNA Prestain Marker Plus are in excellent agreement with sizes of non-stained RNAs, 20, 30, 40, 50, 75 and 100 bases in length (about 95% accuracy). The Small RNA Prestain Marker Plus is supplied in a ready-to-use mixture and doesnt require heating or addition of a denaturing agent before use.
Buffer:
In 2 mM Tris-HCl, pH 8.0 (8 mM EDTA, 78% formamide).
Application Dilute:
Recommended loading volumes:Comb (Load volume)4-10 mm (5-10 uL)>10 mm (>10 uL)
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