Cas9 mRNA is a mature mRNA synthesized in vitro, featuring a 5 Cap 1 structure and a 3 poly(A) tail. It encodes the Cas9 protein, which is equipped with nuclear localization signals (NLS) at both the N- and C-termini to facilitate nuclear entry, thereby enhancing DNA cleavage efficiency. It is an ideal choice for studying transfection and expression through various assay methods.
