Biotinylated Anti-Bevacizumab Antibody (AY13) (recommended for PK/PD), Multiple

Product Overview

• Article Name: Biotinylated Anti-Bevacizumab Antibody (AY13) (recommended for PK/PD), Multiple
• Biozol Catalog Number: ABS-BEB-BY13-25UG
• Supplier Catalog Number: BEB-BY13-25ug
• Alternative Catalog Number: ABS-BEB-BY13-25UG
• Manufacturer: AcroBiosystems
• Host: Multiple
• Category: Antikörper
• Species Reactivity: Mouse
• Conjugation: Biotin

Product Description

A recombinant humanized monoclonal IgG1 antibody that binds to and inhibits the biologic activity of human vascular endothelial growth factor (VEGF). Bevacizumab contains human framework regions and the complementaritydetermining regions of a murine antibody that binds to VEGF. Bevacizumab is produced in a Chinese Hamster Ovary mammalian cell expression system in a nutrient medium containing the antibiotic gentamicin and has a molecular weight of approximately 149 kilodaltons. - Antikörper

Product Properties

• Buffer: PBS, pH7.4
• Purity: 0.95
• Form: Powder
• Target: Bevacizumab

Images

Detection of bevacizumab by bridging ELISA in serum. Immobilized Anti-Bevacizumab Antibody (AY10) (Cat. No. BEB-Y10) at 2 µg/mL, add increasing concentrations of bevacizumab (10% human serum) and then add Biotinylated Anti-Bevacizumab Antibody (AY13) (Cat. No. BEB-BY13) at 2 µg/mL. Detection was performed using HRP-conjugated streptavidin with a sensitivity of 0.4 µg/mL (QC tested).

Immobilized bevacizumab at 1 µg/mL (100 µL/well) can bind Biotinylated Anti-Bevacizumab Antibody (AY13) (Cat. No. BEB-BY13) with a linear range of 0.78-25.5 ng/mL.

Biotinylated Anti-Bevacizumab Antibody (AY13) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 0.95.