GENIUS(TM)Nuclease is a recombinant form of Serratia marcescens extracellular endonuclease produced in Escherichia coli cells using a proprietary process at ACRObiosystems. GENIUS(TM)Nuclease is a homodimer with monomer molecular masses about 30 kDa. Two disulfide bonds found in the nuclease are crucial to its activity and stability. The enzyme is a nonspecific nuclease with high specific activity, which degrades both single and doublestranded nucleic acids in any form ( single stranded, double stranded, linear, circular and supercoiled). It hydrolyzes internal phosphodiester bonds present between the nucleotides to 5' phosphorylated oligonucleotides of 35 bases in length. - Proteine/Peptide
Molecular Weight:
26.8 kDa
Tag:
Native
Buffer:
20mM Tris, 20mM NaCl, 2mM MgCl2, pH8.0
Purity:
95%
Form:
Powder
Target:
Nuclease
Application Notes:
Its high intrinsic activity and broad substrate tolerance make the endonuclease an ideal tool in a variety of biotechnological and pharmaceutical applications: removal of nucleic acid from protein samples ( Elimination of nucleic acids from recombinant proteins, Purification of protein fragments from inclusion bodies, Sample preparation in western blotting or two-dimensional gel electrophoresis) , Viscosity reduction in protein extracts.
BEE-N3116-50KU
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