Biotinylated Human B7-2 / CD86 Protein, Fc,Avitag(TM), premium grade

Product Overview

• Article Name: Biotinylated Human B7-2 / CD86 Protein, Fc,Avitag(TM), premium grade
• Biozol Catalog Number: ABS-CD6-H82F5-200UG
• Supplier Catalog Number: CD6-H82F5-200ug
• Alternative Catalog Number: ABS-CD6-H82F5-200UG
• Manufacturer: AcroBiosystems
• Host: Human
• Category: Proteine/Peptide
• Species Reactivity: Human
• Conjugation: Biotin

Product Description

Cluster of Differentiation 86 (CD86) is also known as Blymphocyte activation antigen B72, is a type I membrane protein that is a member of the immunoglobulin superfamily, and is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B72 is expressed at low levels on monocytes and can be upregulated through interferon gamma. CD86 is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA4 (for attenuation of regulation and cellular disassociation). CD86 works in tandem with CD80 to prime T cells. Recent study has revealed that B72 promotes the generation of a mature APC repertoire and promotes APC function and survival. Furthermore, the B7 proteins are also involved in innate immune responses by activating NFkappaBsignaling pathway in macrophages. CD86 thus is regarded as a promising candidate for immune therapy. CD86+ macrophages in Hodgkin lymphoma patients are an independent marker for potential nonresponse to firstlinetherapy. - Proteine/Peptide

Product Properties

• Molecular Weight: 53.8 kDa
• Tag: C-Fc & C-Avi
• NCBI: 42081
• Buffer: 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5
• Purity: 95%
• Form: Powder
• Target: B7-2

Images

Immobilized Human CTLA-4, Fc Tag (Cat. No. CT4-H5255) at 5 µg/mL (100 µL/well) can bind Biotinylated Human B7-2, Fc,Avitag, premium grade (Cat. No. CD6-H82F5) with a linear range of 19-156 ng/mL (QC tested).

Serial dilutions of Ipilimumab were added into Human CTLA-4, Fc Tag (Cat. No. CT4-H5255): Biotinylated Human B7-2, Fc,Avitag, premium grade (Cat. No. CD6-H82F5) binding reactions. The half maximal inhibitory concentration (IC50) is 0.1701 µg/mL (Routinely tested).

CD6-H82F5-200ug

Biotinylated Human B7-2, Fc,Avitag, premium grade on SDS-PAGE under reducing (R) and under non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.