Biotinylated Human CTLA-4 Protein, His,Avitag(TM) (MALS verified)

Product Overview

• Article Name: Biotinylated Human CTLA-4 Protein, His,Avitag(TM) (MALS verified)
• Biozol Catalog Number: ABS-CT4-H82E3-200UG
• Supplier Catalog Number: CT4-H82E3-200ug
• Alternative Catalog Number: ABS-CT4-H82E3-200UG
• Manufacturer: AcroBiosystems
• Host: Human
• Category: Proteine/Peptide
• Species Reactivity: Human
• Conjugation: Biotin

Product Description

CTLA4 (Cytotoxic TLymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the Tcell costimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B71 and B72 respectively, on antigenpresenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. Fusion proteins of CTLA4 and antibodies (CTLA4Ig) have been used in clinical trials for rheumatoid arthritis. - Proteine/Peptide

Product Properties

• Molecular Weight: 17.2 kDa
• Tag: C-10*His & C-Avi
• NCBI: 16410
• Buffer: PBS, pH7.4
• Purity: 95%
• Form: Powder
• Target: CTLA-4

Images

Immobilized Human B7-1, Fc Tag (Cat. No. B71-H5259) at 2 µg/mL (100 µL/well) can bind Biotinylated Human CTLA-4, His,Avitag (Cat. No. CT4-H82E3) with a linear range of 0.7-13 ng/mL (QC tested).

Quantitative Analysis of CTLA-4 x OX40 Bispecific Antibody in Human Serum by Intact Assay.Immobilized Human OX40 Protein, His Tag (MALS verified) (Cat. No. OX0-H5224) at 2 µg/mL, add increasing concentrations of CTLA-4 x OX40 bispecific antibody in 50% Human serum and then add Biotinylated Human CTLA-4, His, Avitag (Cat. No. CT4-H82E3) at 0.2 µg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 4 ng/mL (Intact assay, Routinely tested).

Biotinylated Human CTLA-4, His,Avitag on SDS-PAGE under reducing (R) and under non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

CT4-H82E3-200ug