HIV-1 [HIV-1/Clade E (CM244)] GP120 Protein, His Tag, Unconjugated, Human

Product Overview

• Article Name: HIV-1 [HIV-1/Clade E (CM244)] GP120 Protein, His Tag, Unconjugated, Human
• Biozol Catalog Number: ABS-GP4-V15223-100UG
• Supplier Catalog Number: GP4-V15223-100ug
• Alternative Catalog Number: ABS-GP4-V15223-100UG
• Manufacturer: AcroBiosystems
• Host: Human
• Category: Proteine/Peptide
• Species Reactivity: Virus
• Conjugation: Unconjugated

Product Description

Human Immunodeficiency Virus (HIV) can be divided into two major types, HIV type 1 (HIV1) and HIV type 2 (HIV2). HIV1 is related to viruses found in chimpanzees and gorillas living in western Africa. HIV2 is related to viruses found in sooty mangabeys. HIV1 viruses may be further divided into groups. The HIV1 group M viruses predominate and are responsible for the AIDS pandemic. Some of the HIV1 group M subtypes are known to be more virulent or are resistant to different medications. HIV2 viruses are thought to be less virulent and transmissible than HIV1 M group viruses. Envelope glycoprotein GP120 (or gp120) is the name of the glycoprotein which forms the spikes sticking out of a HIV virus particle. gp120 is essential for virus entry into cells as it plays a vital role in seeking out specific cell surface receptors for entry. Three gp120s, bound as heterodimers to a transmembrane glycoprotein, gp41, are thought to combine in a trimer to form the envelope spike, which is involved in viruscell attachment. One half of the molecular weight of gp120 is due to the carbohydrate side chains (the glyco in glycoprotein). These are sugar residues which form something almost like a sugar dome over the gp120 spikes. This dome prevents gp120 from being recognised by the human immune response. As the HIV virus and the human CD4 cell come together, the gp120 binding site snaps open at the last minute.The glycoprotein gp120 is anchored to the viral membrane, or envelope, via noncovalent bonds with the transmembrane glycoprotein, gp41. It is involved in entry into cells by binding to CD4 receptors, particularly helper Tcells. Binding to CD4 is mainly electrostatic although there are van der Waals interactions and hydrogen bonds. - Proteine/Peptide

Product Properties

• Molecular Weight: 54.1 kDa
• Tag: C-6*His
• NCBI: 4
• Buffer: PBS, pH7.4
• Purity: 95%
• Form: Powder
• Target: Envelope glycoprotein gp120/gp120 (HIV)

Images

Immobilized Human CD4, Fc Tag (Cat. No. CD4-H5259) at 5 µg/mL (100 µL/well) can bind HIV-1 [HIV-1/Clade E (CM244)] GP120, His Tag (Cat. No. GP4-V15223) with a linear range of 20-156 ng/mL (QC tested).

Immobilized Biotinylated Human CD4, Fc Tag (Cat. No. CD4-H82F3) at 1 µg/mL (100 µL/well) can bind HIV-1 [HIV-1/Clade E (CM244)] GP120, His Tag (Cat. No. GP4-V15223) with a linear range of 0.156-0.313 µg/mL (Routinely tested).

HIV-1 [HIV-1/Clade E (CM244)] GP120, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.