Biotinylated Human IL-8 protein, His,Avitag(TM)

Catalog Number: ABS-IL8-H82H3-25UG
Article Name: Biotinylated Human IL-8 protein, His,Avitag(TM)
Biozol Catalog Number: ABS-IL8-H82H3-25UG
Supplier Catalog Number: IL8-H82H3-25ug
Alternative Catalog Number: ABS-IL8-H82H3-25UG
Manufacturer: AcroBiosystems
Host: Human
Category: Proteine/Peptide
Species Reactivity: Human
Conjugation: Biotin
Interleukin 8 (IL8 or chemokine (CXC motif) ligand 8, CXCL8) is a chemokine produced by macrophages and other cell types such as epithelial cells, airway smooth muscle cells and endothelial cells. There are many receptors on the surface membrane capable of binding IL8, the most frequently studied types are the G proteincoupled serpentine receptors CXCR1 and CXCR2. Expression and affinity for IL8 differs between the two receptors (CXCR1 > CXCR2). IL8(677) has a 510fold higher activity on neutrophil activation, IL8(577) has increased activity on neutrophil activation and IL8(777) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL8(177), respectively. - Proteine/Peptide
Molecular Weight: 12.0 kDa
Tag: C-10*His & C-Avi
NCBI: 10145
Buffer: PBS, pH7.4
Purity: 90%
Form: Powder
Target: IL-8
Immobilized Biotinylated Human IL-8, His,Avitag (Cat. No. IL8-H82H3) at 1 µg/mL (100 µL/well) on streptavidin precoated (0.5 µg/well) plate can bind Monoclonal Anti-Human IL-8 Antibody, Human IgG1 with a linear range of 0.06-2 ng/mL (QC tested).
Immobilized Monoclonal Anti-Human IL-8 Antibody, Human IgG1 at 1 µg/mL (100 µL/well) can bind Biotinylated Human IL-8, His,Avitag (Cat. No. IL8-H82H3) with a linear range of 0.06-0.24 ng/mL (Routinely tested).
Biotinylated Human IL-8, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.