SARS-CoV-2 S protein (L18F, D80A, D215G, 242-244del, R246I, K417N, E484K, N501Y, D614G, A701V) trimer, His Tag (MALS verified), Unconjugated, Human

Product Overview

• Article Name: SARS-CoV-2 S protein (L18F, D80A, D215G, 242-244del, R246I, K417N, E484K, N501Y, D614G, A701V) trimer, His Tag (MALS verified), Unconjugated, Human
• Biozol Catalog Number: ABS-SPN-C52HK-500UG
• Supplier Catalog Number: SPN-C52Hk-500ug
• Alternative Catalog Number: ABS-SPN-C52HK-500UG
• Manufacturer: AcroBiosystems
• Host: Human
• Category: Proteine/Peptide
• Species Reactivity: Virus
• Conjugation: Unconjugated
• Alternative Names: sars-cov-2

Product Description

SARSCoV2 S protein trimer, His Tag (SPNC52Hk) is expressed from human 293 cells (HEK293). It contains AA Val 16 Pro 1213 (Accession QHD43416.1). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the Cterminus. Proline substitutions (F817P/ A892P/ A899P/ A942P/ K986P/ V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARSCoV2 S protein and abolish the furin cleavage site, respectively. The L18F/ D80A/ D215G/ LAL242244del/ R246I/ K417N/ E484K/ N501Y/ D614G/ A701V mutations were identified in the SARSCoV2 Beta variant (Pango lineage: B.1.351, other names: 20H/501Y.V2). - Proteine/Peptide

Product Properties

• Molecular Weight: 137.6 kDa
• Tag: C-10*His
• NCBI: 43416
• Buffer: 0.1 M Sodium citrate, pH5.5
• Purity: 90%
• Form: Powder
• Target: Spike protein

Images

Immobilized SARS-CoV-2 S protein trimer, His Tag (Cat. No. SPN-C52Hk) at 1 µg/mL (100 µL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.4-6 ng/mL (QC tested).

Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 1 µg/mL (100 µL/well) can bind SARS-CoV-2 S protein trimer, His Tag (Cat. No. SPN-C52Hk) with a linear range of 0.2-3 ng/mL (Routinely tested).

SARS-CoV-2 S protein trimer, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SPN-C52Hk-500ug