Biotinylated SARS-CoV-2 S protein, His,Avitag(TM), Super stable trimer (MALS verified), Human

Catalog Number: ABS-SPN-C82E9-200UG
Article Name: Biotinylated SARS-CoV-2 S protein, His,Avitag(TM), Super stable trimer (MALS verified), Human
Biozol Catalog Number: ABS-SPN-C82E9-200UG
Supplier Catalog Number: SPN-C82E9-200ug
Alternative Catalog Number: ABS-SPN-C82E9-200UG
Manufacturer: AcroBiosystems
Host: Human
Category: Proteine/Peptide
Species Reactivity: Virus
Conjugation: Biotin
Alternative Names: sars-cov-2
Biotinylated SARSCoV2 S protein, His, Avitag, Super stable trimer (SPNC82E9) is the ectodomain of SARSCoV2 S protein which contains AA Val 16 Pro 1213 (Accession QHD43416.1). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the Cterminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARSCoV2 S protein and abolish the furin cleavage site, respectively. - Proteine/Peptide
Molecular Weight: 139.7 kDa
Tag: C-10*His & C-Avi
NCBI: 43416
Buffer: 0.1 M Sodium citrate, pH5.5
Purity: 95%
Form: Powder
Target: Spike protein
Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 1 µg/mL (100 µL/well) can bind Biotinylated SARS-CoV-2 S protein, His,Avitag, Super stable trimer (Cat. No. SPN-C82E9) with a linear range of 0.004-0.125 µg/mL (QC tested).
Immobilized Biotinylated SARS-CoV-2 S protein, His,Avitag, Super stable trimer (Cat. No. SPN-C82E9) at 1 µg/mL (100 µL/well)on Recombinant Streptavidin (Cat. No. STN-N5116) precoated (0.5 µg/well) plate. can bind Anti-SARS-CoV-2 RBD Neutralizing Antibody, Human IgG1 (Cat. No. SAD-S35) with a linear range of 0.1-2 ng/mL (Routinely tested).
Biotinylated SARS-CoV-2 S protein, His,Avitag, Super stable trimer on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
SPN-C82E9-200ug