Biotinylated SARS-CoV-2 Spike Trimer Protein (T19R, V70F, FR157-158Del, A222V, W258L, K417N, L452R, T478K, D614G, P681R, D950N), His,Avitag(TM) (MALS verified), Human

Product Overview

• Article Name: Biotinylated SARS-CoV-2 Spike Trimer Protein (T19R, V70F, FR157-158Del, A222V, W258L, K417N, L452R, T478K, D614G, P681R, D950N), His,Avitag(TM) (MALS verified), Human
• Biozol Catalog Number: ABS-SPN-C82EI-25UG
• Supplier Catalog Number: SPN-C82Ei-25ug
• Alternative Catalog Number: ABS-SPN-C82EI-25UG
• Manufacturer: AcroBiosystems
• Host: Human
• Category: Proteine/Peptide
• Species Reactivity: Virus
• Conjugation: Biotin
• Alternative Names: sars-cov-2

Product Description

Biotinylated SARSCoV2 Spike Trimer, His,Avitag (SPNC82Ei) is the ectodomain of SARSCoV2 spike protein which contains AA Val 16 Pro 1213 (Accession QHD43416.1). Mutations T19R, V70F, FR157158Del, A222V, W258L, K417N, L452R, T478K, D614G, P681R, D950N were identified in the SARSCoV2 Delta sublineages (Pango lineage: AY.1/AY.2/AY.3, also known as Delta Plus). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the Cterminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARSCoV2 S protein and abolish the furin cleavage site, respectively. - Proteine/Peptide

Product Properties

• Molecular Weight: 139.5 kDa
• Tag: C-10*His & C-Avi
• NCBI: 43416
• Buffer: 0.1 M Sodium citrate, pH5.5
• Purity: 95%
• Form: Powder
• Target: Spike protein

Images

Immobilized Biotinylated SARS-CoV-2 Spike Trimer, His,Avitag (Cat. No. SPN-C82Ei) at 1 µg/mL (100 µL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 µg/well) plate can bind Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (Cat. No. S1N-M122) with a linear range of 0.1-2 ng/mL (QC tested).

Immobilized Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (Cat. No. S1N-M122) at 1 µg/mL (100 µL/well) can bind Biotinylated SARS-CoV-2 Spike Trimer, His,Avitag (Cat. No. SPN-C82Ei) with a linear range of 0.8-25 ng/mL (Routinely tested).

Biotinylated SARS-CoV-2 Spike Trimer, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.