Human Tau-441 K18 (P301L) Pre-formed Fibrils Protein, Tag Free, Unconjugated, E. coli
Biozol Catalog Number:
ABS-TAU-H5113-500UG
Supplier Catalog Number:
TAU-H5113-500ug
Alternative Catalog Number:
ABS-TAU-H5113-500UG
Manufacturer:
AcroBiosystems
Host:
E. coli
Category:
Proteine/Peptide
Species Reactivity:
Human
Conjugation:
Unconjugated
This gene encodes the microtubuleassociated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimers disease, Picks disease, frontotemporal dementia, corticobasal degeneration and progressive supranuclear palsy. [provided by RefSeq, Jul 2008] - Proteine/Peptide
1. Sonication Conditions, Dissolution, Aliquoting, Storage, and Notes for PFF Thawing: Thaw PFFs rapidly in a 37 C water bath, or allow to thaw at room temperature. Aliquoting: Since PFFs are supplied as a suspension, pipette up and down thoroughly before aliquoting to ensure homogeneity. Storage: Store at -80 C at all times, avoid storage at 4 C or -20 C, which can induce fibril depolymerization. * alpha-Syn fibrils cold-denatured to monomers at 0-20 C and heat-denatured at 60-110 C. Sonication: The sonication protocols below are based on cell-based assay conditions. -Option 1 (Probe Sonicator): Use a probe sonicator (SCIENTZ) at 10% power (-95 W), applying 60 pulses of 0.5 s on/0.5 s off (recommended). -Option 2 (Ultrasonic Bath): Sonicate in an ultrasonic cleaner (40 kHz, 200-400 W) at 37 C for 1 hour, avoid performing the treatment at 4 C or 20 C. Note: The above sonication protocols are based on cell-based assays, other applications (e.g., in vivo injections) may require optimization-users should determine their optimal settings for their specific use case. 2. Recommendations for PFF Use in Animal Models Minimize Freeze-Thaw Cycles: For animal studies, it is recommend to use the PFF at once or aliquot before use to avoid repeated freeze-thaw cycles. PFF Maintenance During Injections: During stereotaxic injections, keep the sonicated PFFs in a 37 C water bath to prevent re-aggregation or sedimentation.
TAU-H5113-500ug
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