Human Thrombopoietin / TPO Protein, premium grade, Unconjugated
Biozol Catalog Number:
ABS-THN-H5216-1MG
Supplier Catalog Number:
THN-H5216-1mg
Alternative Catalog Number:
ABS-THN-H5216-1MG
Manufacturer:
AcroBiosystems
Host:
Human
Category:
Proteine/Peptide
Species Reactivity:
Human
Conjugation:
Unconjugated
Thrombopoietin (Tpo), is a key regulator of megakaryocytopoiesis and thrombopoiesis. It is principally produced in the liver and is bound and internalized by the receptor Tpo R/cmpl. Defects in the TpoTpo R signaling pathway are associated with a variety of platelet disorders (13). The 353 amino acid (aa) human Tpo precursor is cleaved to yield the 332 aa mature protein. Mature human Tpo shares approximately 70% aa sequence homology with mouse and rat Tpo. It is an 80-85 kDa protein that consists of an N-terminal domain with homology to Erythropoietin (Epo) and a C-terminal domain that contains multiple N-linked and Olinked glycosylation sites (4, 5). Tissue specific alternate splicing of human Tpo generates multiple isoforms with internal deletions, insertions, and/or C-terminal substitutions (6). Tpo promotes the differentiation, proliferation, and maturation of MK and their progenitors (4, 5, 7). Several other cytokines can promote these functions as well but only in cooperation with Tpo (8, 9). Notably, IL3 independently induces MK development, although its effects are restricted to early in the MK lineage (8, 9). Tpo additionally promotes platelet production, aggregation, ECM adhesion, and activation (1013). It is cleaved by plateletderived thrombin following Arg191 within the C-terminal domain and subsequently at other sites upon extended digestion (14). Both full length Tpo and shorter forms circulate in the plasma, with the shorter, N-terminal EPOlike domain forms showing significantly increased specific activity (4, 5, 15). The C-terminal domain is not required for binding to Tpo R or inducing MK growth and differentiation (5). Aside from its hematopoietic effects, Tpo is expressed in the brain where it promotes the apoptosis of hypoxiasensitized neurons and inhibits neuronal differentiation by blocking NGF induced signaling (16, 17). - Proteine/Peptide
