SARS-CoV-2 Spike Antibody (AM020553)

Catalog Number: ACM-91381
Article Name: SARS-CoV-2 Spike Antibody (AM020553)
Biozol Catalog Number: ACM-91381
Supplier Catalog Number: 91381
Alternative Catalog Number: ACM-91381
Manufacturer: Active Motif
Category: Antikörper
Alternative Names: sars-cov-2

Antibody Type:

Recombinant

Isotype:

IgG1

Purification:

Protein A Chromatography

Host:

Human

Molecular Weight:

141 kDa (full length S1 protein)

Reactivity:

Virus

Applications

Enzyme-linked Immunosorbent Assay Validated

Application Notes

Note: Clone AM020553, developed by Active Motif, is also referred to as Clone 553-20.
Details on the development and characterization of this clone are available here: Wan J., et. al. Human IgG neutralizing monoclonal antibodies block SARS-CoV-2 infection. Cell Reports, 32: 107918, July 21, 2020.

The antibody has been tested by ELISA and is specific for SARS-CoV-2 Spike protein.

Recommended Range:
ELISA: 0.01 - 1 µg/ml

Immunogen

N/A - derived from COVID-19 patients who have cleared the virus. Their antibodies were screened for reactivity to SARS-CoV-2 and then sequenced and expressed as recombinant antibodies.

Buffer

140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, and 30% glycerol.

 

SARS-CoV-2 Spike Antibody (AM020553) tested by ELISA.

AbFlex SARS-CoV-2 Spike Antibody (rAb) (AM020553) tested by ELISA using SARS Spike protein ECD.
SARS-CoV-2 Spike Extracellular Domain (ECD a.a. 14-1213) protein was coated onto microtiter plates at 5 µg/mL and then incubated with a dilution series of SARS-CoV-2 Spike Antibody (rAb) (clone AM020553). Bound antibodies were detected with anti-human IgG conjugated to horseradish peroxidase (HRP) followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm.

Background

COVID-19, which is short for coronavirus disease 2019, is the official name of the respiratory disease caused by infection with the novel coronavirus SARS-CoV-2. The virus that causes COVID-19 was named SARS-CoV-2 because it is a coronavirus genetically similar to, yet distinct from, the virus that caused the severe acute respiratory syndrome (SARS) outbreak in 2003. Studying the details of how this virus replicates and causes the disease will allow scientists and physicians to more rapidly develop fast and accurate methods of detection as well as to deploy therapeutic and vaccine strategies.

This antibody was derived from COVID-19 patients who have cleared the virus. Patient serum IgG was sequenced and expressed as full-length IgG1 with human immunoglobulin heavy and light chains in mammalian 293 cells.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.