Anti-H+ATPase | Plasma membrane H+ATPase (rabbit antibody), Biotin conjugated (), Rabbit, Polyclonal

Product Overview

• Article Name: Anti-H+ATPase | Plasma membrane H+ATPase (rabbit antibody), Biotin conjugated (), Rabbit, Polyclonal
• Biozol Catalog Number: AGR-AS07-260B
• Supplier Catalog Number: AS07-260B
• Alternative Catalog Number: AGR-AS07-260B
• Manufacturer: Agrisera
• Host: Rabbit
• Category: Antikörper
• Application: IL, WB
• Species Reactivity: A. thaliana, Plant
• Immunogen: KLH-conjugated synthetic peptide, derived from available di and monocot, fern, mosses and algal plasma membrane ATPase sequences including Arabidopsis thaliana ATPase 1 (UniProt: P20649, TAIR: At2g18960) and ATPase 2 (UniProt: P19456 , TAIR: At4g30190), 3 (UniProt: P20431, TAIR: At5g57350), 4 (UniProt: Q9SU58, TAIR: At3g47950), 6 (UniProt: Q9SH76, TAIR: At2g07560), 7 (UniProt: Q9LY32, TAIR: At3g60330), 8 (UniProt: Q9M2A0, TAIR: At3g42640), 9 (UniProt: Q42556, TAIR: At1g80660), 11 (UniProt: Q9LV11, TAIR: At5g62670) of Arabidopsis thaliana and hydrogen ATPase of Chlamydomonas reinhardtii (Q9FNS3)

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 90- 95 kDa (Arabidopsis thaliana, depending upon an isoform)
• UniProt: P20649
• Purity: Antigen affinity purified serum in PBS pH 7.4, conjugated to Biotin.
• Form: Liquid

Application Notes

• Application Dilute: 1 : 100 (IL), 1 : 1000-1 : 5 000 (WB)
• Application Notes: VERY IMPORTANT: Please, do not heat up your samples above 70C as this may cause H+ATPase to precipitate, and there will be no signal on your Western Blot. Before SDS-PAGE, centrifuge your samples at room temperature at 10 000 rpm/1 min to remove any aggregates. H+ATPase will be less abundant in mature roots and leafs, and therefore detection may require use of very sensitive reagents.