PsbD | D2 protein of PSII positive control/quantitation standard
Biozol Catalog Number:
AGR-AS09-146S
Supplier Catalog Number:
AS09-146S
Alternative Catalog Number:
AGR-AS09-146S
Manufacturer:
Agrisera
Category:
Proteine/Peptide
Application:
WB
D2 protein (PsbD) forms the reaction core of PSII (Photosystem II) as a heterodimer with the D1 protein (PsbA). PsbD is homologous to the D1 protein, with slightly higher molecular mass of about 39,5 kDa. Accumulation of D2 protein is an important step i
Molecular Weight:
In most gel systems PsbD migrates around 28-30 kDa
Form:
Lyophilized in glycerol
Antibody Type:
Secondary Antibody
Application Dilute:
Standard curve: 3 loads are recommended (0.5, 2 and 4 µl).For most applications a sample load of 0.2 µg of chlorophyll will give a PsbD signal in this range.Positive control: a 2 µl load per well is optimal for most chemiluminescent detection systems. Thi
Application Notes:
Concentration: after adding 225 µl of milliQ water final concentration of the standard is 0.25 pmoles/ulProtein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1 mg/ml PefaBloc protease inhibitor (Roche), 50 mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
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