5 µg of antibody per 1 gram of a fresh tissue (IP), 1 : 10 000 (WB)
Application Notes:
AGO expression may be cell/tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Seedlings can be used as a negative control. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.A recommended whole-mount immunolocalization protocol can be found here.
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