Synthetic HDEL peptide corresponding to the C-terminus of yeast Bip protein
HDEL (his-asp-glu-leu) is a C-terminal tertapeptide found in yeast and plants which allows sorting of resident soluble proteins in the lumen of the endoplasmic reticulum.
Clonality:
Monoclonal
Molecular Weight:
78 kDa
Isotype:
IgG2b
Purity:
Total IgG. Protein G purified in PBS pH 7.4.
Form:
Liquid
Antibody Type:
Monoclonal Antibody
Application Dilute:
1: 50-1 : 500 (IF), 1 : 100-1, 1000 (WB)
Application Notes:
Antibody in concentration 1 µg/ml was sufficient for detection of HDEL-containing proteins in 10 µg of yeast cell lysate by colorimetric western blot. Clone 2E7. For western blot results, immunofluorescence and immunogold images please referr to Napier et al. 1992.
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