KLH-conjugated peptide derived from Arabidopsis thaliana ELF3, UniProt: O82804-1,TAIR: At2g25930
ELF3 (Early Flowering 3) is a transcription factor, which acts within a zeitnehmer feedback loop and is involved in its own circadian regulation and is a part of a corepressor complex consisting of ELF4, ELF3, and LUX involved in the transcriptional regu
3 µl of antibody (ChIP), 3 µl of antibody used for coating 2x20 µl Dynabeads A+G (IP), 1 : 1000 (WB)
Application Notes:
ELF3 protein is extremely sensitive to light therefore extraction has to be done in green light and protein analysis has to be done using fresh extracts only. Even short light exposure cause degradation of ELF3. Extracted protein cannot be frozen but analysed directly following extraction.Antibody is confirmed to work in ChIP for Oryza sativa Nippon bare.Antibody is recognizing all three isoforms in tomato: Solyc08g065870.4.1: Theoretical pI/Mw: 8.71 / 70659.09 DaSolyc11g070100.2.1: Theoretical pI/Mw: 8.94 / 66714.77 DaSolyc12g095900.2.1: Theoretical pI/Mw: 8.80 / 79108.57 Da
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