Anti-TIP1,1, TIP1,2 | tonoplast intrinistic protein 1-1, 1-2 (gamma), Rabbit, Polyclonal

Product Overview

• Article Name: Anti-TIP1,1, TIP1,2 | tonoplast intrinistic protein 1-1, 1-2 (gamma), Rabbit, Polyclonal
• Biozol Catalog Number: AGR-AS22-4844
• Supplier Catalog Number: AS22-4844
• Alternative Catalog Number: AGR-AS22-4844
• Manufacturer: Agrisera
• Host: Rabbit
• Category: Antikörper
• Application: WB
• Species Reactivity: A. thaliana, Plant
• Immunogen: KLH-conjugated synthetic peptide conserved in Raphanus sativus TIP1,1 and TIP1,2 (protein accesion number available in Suga et al. 2001). Peptide is also conserved in Arabidopsis thaliana TIP1-1 P25818, At2g36830, TIP1-2 Q41963, At3g26520

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 25.8 | 23 kDa
• NCBI: 818255
• UniProt: P25818
• Purity: Antigen affinity purified serum, in PBS pH 7.4
• Form: Lyophilized

Application Notes

• Application Dilute: 1 : 1000 (WB)
• Application Notes: Protein or membrane sample should be treated at 70C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20C to -80C.Triton X-100 should not be included in the protein extraction buffer, when cell organelles or membrane proteins must be separated from soluble proteins. Because, Triton X breaks membrane structure and solubilizes most membranes proteins. Furthermore, it should be noted that Triton X at high concentrations binds SDS and mask the detergent effect of SDS for SDS-PAGE. Also, micelles of Triton X behave as a large complex with molecular mass of 90 kDa at high concentrations in SDS-PAGE.