Rhodamine B hydrazide is a good sulfite probe with colorless, non-fluorescent properties, which produces fluorescence intensity dependent on sulfite concentration (5-800 ng/mL, detection limit = 1.4 ng/mL (3sigma)). Sulfite reduction solubilizes oxygen and generates superoxide radicals, which bind to Rhodamine B hydrazide to form the fluorescent product Rhodamine B. This fluorescence can be enhanced by Tween 80 surfactant micelles.Rhodamine B hydrazide exhibits maximum absorption at 554 nm and maximum fluorescence emission at 574 nm. Rhodamine B hydrazide has maximum absorption at 554 nm and maximum fluorescence emission at 574 nm.
Molecular Weight:
456.58
Purity:
99.0%
CAS Number:
[74317-53-6]
Formula:
C28H32N4O2
Target:
Fluorescent Dye
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