2,7-Dichlorodihydrofluorescein (DCFH2) is a non-fluorescent reactive oxygen species (ROS) probe with an excitation wavelength of 485-500 nm and an emission wavelength of 515-530 nm. 2,7-Dichlorodihydrofluorescein is first hydrolyzed by intracellular esterases and then oxidized by ROS to generate non-biomembrane-permeable, highly fluorescent 2,7-dichlorofluorescein (DCF). 2,7-Dichlorodihydrofluorescein can undergo oxidation reactions with a variety of ROS (such as OH, H2O2, ONOO-, etc.) and is used to quantitatively detect the level of oxidative stress inside and outside cells. The fluorescence intensity of DCF is positively correlated with the ROS concentration.
Molecular Weight:
403.21
Purity:
99.0%
CAS Number:
[106070-31-9]
Formula:
C20H12Cl2O5
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