CCNB1 Antibody - C-terminal region

Product Overview

• Article Name: CCNB1 Antibody - C-terminal region
• Biozol Catalog Number: ASB-ARP30161_P050
• Supplier Catalog Number: ARP30161_P050
• Alternative Catalog Number: ASB-ARP30161_P050-25UL,ASB-ARP30161_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IP, WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human CCNB1
• Alternative Names: CCNB

Product Description

CCNB1 is a regulatory protein involved in mitosis. CCNB1 complexes with p34(cdc2) to form the maturation-promoting factor (MPF).The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 48kDa
• NCBI: 891
• UniProt: P14635
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Rabbit Anti-CCNB1 Antibody
Catalog Number: ARP30161_P050
Formalin Fixed Paraffin Embedded Tissue: Human Lymph Node Tissue
Observed Staining: Cytoplasm
Primary Antibody Concentration: 1:600
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

Researcher: Dr. Yuzhi Chen, University of Arkansas for Medical Science
Application: IP
Species+tissue/cell type: Mouse brain homogenate
How many ugsof tissue/cell lysate run on the gel: 500 ug mouse brain homogenateIP antibody: 6 ug
Primary antibody dilution: 1:500
Secondary antibody: Goat anti-rabbit Alexa-Fluor 594
Secondary antibody dilution: 1:5000

Researcher:Dr. Yuzhi Chen, University of Arkansas for Medical Science
Application:Western blotting
Species+tissue/cell type: lane 1: 60ug human NT2 cell line
Primary antibody dilution: 1:500
Secondary antibody:IRDye 800 CW goat anti-rabbit from Li-COR Bioscience
Secondary antibody dilution:1:20,000

WB Suggested Anti-CCNB1 Antibody Titration: 0.125ug/ml
ELISA Titer: 1:312500
Positive Control: Jurkat cell lysateCCNB1 is supported by BioGPS gene expression data to be expressed in Jurkat

WB Suggested Anti-CCNB1 Antibody
Positive Control: Lane 1: 60ug human NT2 cell line
Primary Antibody Dilution : 1:500
Secondary Antibody : IRDye 800 CW goat anti-rabbit from Li-COR Bioscience
Secondry Antibody Dilution : 1:20,000
Submitted by: Dr. Yuzhi Chen, University of Arkansas for Medical Science

CCNB1 antibody - C-terminal region (ARP30161_P050) in Human Jurkat using Western Blot