Lanes: 1. 30 ug human HCT116 cell lysate 2. 30 ug genotoxic treated human HCT116 cell lysate 3. 30 ug genotoxic treated human HCT116 cell lysate Primary Antibody Dilution: 1:2000 Secondary Antibody: Anti-rabbit HRP Secondary Antibody Dilution: 1:5000 Gene Name: CDK4 Submitted by: Anke Rauch & Dr. Oliver Kramer, Institute for Biochemistry and Biophysics, Friedrich-schiller University Jena CDK4 is supported by BioGPS gene expression data to be expressed in HCT116
Lanes: Lane1: 30 ug untreated human HCT116 cell lysate Lane2-3: 30 ug genotoxic agent treated human HCT116 cell lysate Primary Antibody Dilution: 1:2000 Secondary Antibody: Anti-rabbit HRP Secondary Antibody Dilution: 1:5000 Gene Name: CDK4 Submitted by: Anke Rauch & Dr. Oliver Kramer, Institute for Biochemistry and Biophysics, Center for Molecular Biomedicine, Friedrich-Schiller University Jena CDK4 is supported by BioGPS gene expression data to be expressed in HCT116
CDK4 was immunoprecipitated from 2 mg HEK293 Whole Cell Lysate with ARP30259_P050 with 1:200 dilution. Western blot was performed using ARP30259_P050 at 1/1000 dilution. Lane 1: Control IP in HEK293 Whole Cell Lysate. Lane 2: CDK4 IP with ARP30259_P050 in HEK293 Whole Cell Lysate. Lane 3: Input of HEK293 Whole Cell Lysate.